Diversidad de bacterias totales y endófitas asociadas a raíces del pasto bothriochloa pertusa (l) a. Camus
Diversity of bacteria total and endophytes associated grass roots bothriochloa pertusa (l) a. Camus

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Autores

ALEXANDER. PEREZ C

JOHANNA ROJAS S

JUSTO FUENTES C

Resumen

El objetivo del presente trabajo fue determinar la diversidad de comunidades cultivables de bacterias totales y endófitas asociadas a raíces del pasto colosuana en el departamento de Sucre. La diversidad de comunidades de bacterias totales y endófitas fue realizada mediante aislamiento de colonias en medios de cultivos (SAKIYAMA et al., 2001). La densidad poblacional fue estimada por conteo directo de colonias en placa y las características culturales de cada morfotipo fueron obtenidas mediante observación de cada colonia formada. Una vez cuantificadas las comunidades de bacterias totales y endófitas, se determinó la riqueza de especie (R), el índice de diversidad de ShannonWeaver (H) y la equitabilidad de morfotipos mediante modelos matemáticos propuestos para cada fin. Para establecer diferencias estadísticas entre comunidades de bacterias totales y endófitas se realizaron ANOVAS, test paramétricos y no paramétricos utilizando el programa R. Fueron muestreadas 14 fincas ganaderas, localizadas en la región fisiográfica de Sabanas de Sucre, donde fue reportada la presencia por primera vez de bacterias endófitas asociadas a las raíces de colosuana. Fueron encontradas diferencias significativas entre la riqueza de especies y la equitabilidad de bacterias totales y endófitas en raíces del pasto. Este es el primer reporte que se tiene sobre la presencia de bacterias endófitas en la raíces de este pasto a nivel mundial.

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Referencias

AGUILERA, M.M. 2005. Documento de trabajo sobre economía regional. Economía Regional: La Economía del Departamento de Sucre: Ganadería y Sector Público. Banco Ganadero, 63:50-54.

ARAUJO, W.L.; MARCON, J.; MACCHERONI, W.JR.; VAN ELSAS, J.D.; VAN VUURDE, J.W.L.; AZEVEDO, J.L. 2002. Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Applied Environmental Microbiology 68:4906-4914.

BACON, W.C.; HINTON, D.M.1997. Isolation and culture of endophytic bacteria and fungi: HURST, C. et al. Manual of environmental microbiology. American Society for Microbiology, USA.

BELL, C.R.; DICKIE, G.A.; HARVEY, W.L.; CHAIN, J.M. 1995. Endophytic bacteria in grapevine. Canadian Journal of Microbiology 41(1):46-53.

BERG, G.; KRECHEL, A.; DITZ, M.; SIKORA, A.; ULRICH, A.; HALLMANN, J. 2005. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi, FEMS Microbiology Ecology 5(1):215–229

BROOKS, D.S.; GONZALEZ, C.F.; APPEL, D.N.; FILER, T.H. 1994. Evaluation of endophytic bacteria as potential biological control agents for oak wilt. Biology Conservation 4:373–381.

CANKAR, K.; KRAIGHER, H.; RAVNIKAR, M.; RUPNIK, M. 2005. Bacterial endophytes from seeds of Norway spruce (Picea abies L. Karst). FEMS Microbiology Letters 244(2):341-345.

CHANWAY, C.P. 1998. Bacterial endophytes: ecological and practical implications. Sydowia 50: 149–170.

ESTRADA, P.; MAVINGUI, P.; COURNOYER, B.; FONTAINE, F.; BALANDREAU, J.; CABALLERO-MELLADO, J. 2002. A N2-fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico. Canadian Journal of Microbiology 48:285–294.

GREEN, J.; BOHANNAN, B. 2006. Spatial scaling of microbial biodiversity. Trends in ecology and evolution 21(9): 501-507.

HALLMANN, J.; QUADT-HALLMANN, A.; MAHAFFEE, W.F.; KLOEPPER, J.W. 1997. Bacterial endophytes in agricultural crops. Canadian Journal Microbiology 43:895-914.

HUA-WEI, Z.; YOUG, CH.S.; REN, X.T. 2006. Biology and chemistry of endophytes. Natural Product Reports 23:753-771.

HUANG, J. 1991. Ultrastructure of bacterial penetration in plants. Annual Review of Phythopatology 24:141-157

HUREK, T.; REINHOLD-HUREK, B.; VAN M.; KELLENBERGER, E. 1994. Root colonization and systemic spreading of Azoarcus sp. Strain BH72 in grasses. Journal of Bacteriology 176:1913-1923.

HUREK T., REINHOLD-HUREK B. 2003. Azoarcus sp. strain BH72 as a model for nitrogen-fixing grass endophytes. Journal of Biotechnology 106(2-3):169–178.

JACOBS, M.J.; BUGBEE, W.M., GABRIELSON, D.A. 1985. Enumeration, location, and characterization of endophytic bacteria within sugar beet roots. Canadian Journal of Botany 63:1262-1265.

JIMÉNEZ-SALGADO, T.; FUENTES-RAMIREZ, L.E.; TAPIA-HERNANDEZ, A.; MASCARUA-ESPARZA, M.A.; MARTINEZ-ROMERO, E.; CABALLEROMELLADO, J. 1997. Coffea arabica l., a new host plant for Acetobacter diazotrophicus,and isolation of other nitrogen-fixing acetobacteria. Applied Environmental Microbiology 63:3676-3683

KUKLINSKY-SOBRAL, J.; ARAUJO, W.L.; MENDES, R.; GERALDI, I.O.; PIZZIRANI-KLEINER, A.A.; AZEVEDO, J.L. 2004. Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion. Environmental Microbiology 6:1244-1251

LACAVA, T.P.; DINI, A.F.; ARAUJO, W.; AZEVEDO, J. 2006. Caracterização da comunidade de bactérias endofíticas de cítricos por isolamento, PCR especifica e DGGE. Pesquisa Agropecuária Brasileira 41: 637-542

LAMB, T.G.; TONKYN, D.W.; KLUEPFEL, D.A. 1996. Movement of Pseudomonas aureofaciens from rhizosphere to aerial plant tissue. Canadian Journal of Microbiology 42:1112-1120.

LONG, H.H.; FURUYA, N.; KUROSE, D.; TAKESHITA, M.; TAKANAMI, Y. 2003. Isolation of endophytic bacteria from Solanum sp. and their antibacterial activity against plant pathogenic bacteria. Journal of the Faculty of Agriculture 48:21–28.

MARON, P.; RANJARD, L.; MOUGEL, C.; LEMANCEAU, P. 2006. Metaproteomics: A new Approach for Studying Functional Microbial Ecology. Microbial Ecology 53:486-493.

MOORE F.P.; BARAC T.; BORREMANS B.; OEYEN L.; VANGRONSVELD J.; VAN DER LELIE D.; CAMPBEL C. D.; MOORE, E.R.B. 2006. Endophytic bacterial diversity in poplar trees growing on a BTEX-contaminated site: The characterisation of isolates with potential to enhance phytoremediation. Systematic and Applied Microbiology 29:539–556.

NEWMAN, L.A., REYNOLDS, C.M. 2005. Bacteria and phytoremediation: new uses for endophytic bacteria in plants. Trends in Biotechnology 23(1):6-8.

PILLAY, V.K.; NORWARK J. 1997. Inoculam, density, temperature, and genetype effect on in vitro growth promotion and epiphytec and endophytec colonization of tomato (Lycopersicon esculentum L.), seeding inoculated with a Pseudomonal bacterium. Canadian Journal of Microbiology 43:354-361.

PRESTON, G.; HAUBOLD, B.; RAINEY, P. 1998. Bacterial genomics and adaptation to life plants: implications for the evolution of pathogenicity and symbioses. Current Opinion in Microbiology 45:589-597.

R DEVELOPMENT CORE TEAM R: A Language and Environment for Statistical computing, R Foundation for Statistical Computing, en línea 2009, disponible en:http://www.R-project.org

RANJARD, L.; POLY, F.; NAZARET, S. 2000. Monitoring complex bacterial communities using culture-independente molecular techniques: Application to soil environmental. Research Microbiology 151(3):167-177.

SAKIYAMA, C.C.H.; PAULA, E.M.; PEREIRA, P.C.; BORGES, A.C., SILVA D.O. 2001. Characterization of pectin lyase produced by an endophytic strain isolated from coffee cherries, Letter in Applied Microbiology 33(2):117-121.

SCOTT, B. 2001. Epichloë endophytes: fungal symbionts of grasses. Current Opinion in Microbiology 4(4):393–398.

SESSITSCH, A.; REITER, B.; PFEIFER, U.; WILHELM, E. 2002. Cultivationindependent population analysis of bacterial endophytes in three potato varieties based on eubacterial and Actinomycetes-specific PCR of 16S rRNA genes. FEMS Microbiology Ecology 39(1) :23-32.

SHANNON, C.E.; WEAVER, W. 1949. The mathematical theory of communication. University of Illinois Press. Urbana, IL, EEUU.

SHIOMI, H.; ALVES, S.H.; SOARES, I.; VIEIRA, F.; WAGNER, B. 2006. Bioprospecting Endophytic Bacteria for Biological Control of Coffee leaf rust. Sci. Agric. 63 (1):32-39

SIERRA, O.; BEDOYA, J.A.; MONSALVE, D.; OROZCO, J.J. 1997. Observaciones sobre colosuana (Bothriochloa pertusa (L) Camus) en la costa Atlántica de Colombia. Boletín Pasturas Tropicales 8 (1):1-4.

STROBEL G.A. 2003. Endophytes as sources of bioactive products. Microbes and Infection 5(6):535–544.

TAN, R.X.; ZOU X.W. 2001. Endophytes: A Rich source functional de metabolites. Nature Products 18:448 -459

TÓTOLA, M. R.; CHAER, G. M. 2002. Microrganismos e processos microbiológicos como indicadores da qualidade do solo. Tópicos em Ciências do solo, Sociedade Brasileira de Ciências do Solo 2:25-32.

TSAVKELOVA, E.A.; CHERDYNTSEVA, T.A.; BOTINA, S.G.; NETRUSOV A.I. 2007. Bacteria associated with orchid roots and microbial production of auxin. Microbiological Research 162(1):69-76.

VAN, A. 2004. Biodegradation of Nitro-Substituted Explosives 2,4,6-Trinitrotoluene, Hexahydro-1,3,5-Trinitro-1,3,5-Triazine, and Octahydro-1,3,5,7-Tetranitro-1,3,5- Tetrazocine by a Phytosymbiotic Methylobacterium sp. Associated with Poplar Tissues (Populus deltoides). Applied Environmental Microbiology 70(1): 508–517.

VAN DER LELIE, D.; BARAC, T.; TAGHAVI S.; VANGRONSVELD, J. 2005. Response to Newman. New uses of endophytic bacteria to improve phytoremediation. Trends in Biotechnology 23 (1):8-12.

VEGA, F.; PAVA, R.M.; POSADA, F.; BUYER, J. 2005. Endophytic bacteria in coffea arábica L. Journal Basic of Microbiology 45(5):371-380.

VILORIA, H.J. 2002. Documento de trabajo sobre economía regional: La ganadería bovina en las llanuras del Caribe Colombiano. Banco de la República, Cartagena de Indias.

WARD, DA.2006. A Macrobiological perspective on microbial species. Microbe 1(6):269-278.

WHITESIDES, S.K.; SPOTTS, R.A. 1991. Frequency, distribution, and characteristics of endophytic Pseudomonas syringe in pear trees. Phytopathology 81(4):453–457.

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