Maturação nuclear in vitro de ovócitos bovinos selecionados pelo método azul cresil brilhante

Rafael Otero A, Eduardo Da Costa P, Emílio Pereira M

Resumen


Objetivou-se investigar a competência in vitro da maturação nuclear de ovócitos selecionados pela técnica do corante  Azul Cresil Brilhante (ACB), após manutenção em meio Talp-Hepes. Complexos cumulus ovócito (CCOs) foram obtidos de ovários de matadouro e distribuídos aleatoriamente em 6 tratamentos. Quanto percentual de ovócitos que apresentaram configuração nuclear de MI (Metáfise I) ao término da MIV, notou-se que T3 e T5 apresentaram-se superiores ao T1, T2 e T4 (p<0,05). Quanto à capacidade dos ovócitos de completarem a MIV, expresso pela competência em atingirem MII (Metáfise II), observou-se que os tratamentos T1, T2, T4 apresentaram maior percentual em MII que T3 e T5 (p<0,05). Dos ovócitos submetidos a MIV, T1 apresentou menor percentual de degenerados que T2, T3, T4, T5 (p<0,05). Concluiu-se que a capacidade de coloração de ovócitos por ACB não alterou após manutenção dos CCOs por 5 horas em Talp-Hepes e que o corante ACB mostrou-se eficaz na seleção de ovócitos mais competentes após manutenção de CCOs por 5h em Talp-Hepes.

Palabras clave


Ovócitos; Azul cresil brilhante; Maturação in vitro.

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Referencias


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DOI: https://doi.org/10.24188/recia.v9.n2.2017.617

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